Effects of Kolaviron on the Histoarchitecture of the Ovary, Fallopian Tube and Uterus in Female Wistar Rats

Abstract

This research work determined the effects of kolaviron (a methanolic extract of Garcinia kola seeds) on the histoarchitectures of ovary, fallopian tube and uterus with a view to access its relationship to infertility. A total of thirty adult female Wistar rats were used for this experiment. The animals were randomly divided into six (6) groups: A, B, C, D, E and F with five (5) animals in each group. Group A, were the control group that were given distilled water orally once daily for 14 days; Group B were given kolaviron orally at concentration of 200 mg/kg body weight once daily for 14 days; Group C were given kolaviron orally at concentration of 100 mg/kg twice daily for 14 days; Group D were given clomiphene citrate orally at concentration of 0.50 mg/kg body weight for 5 days; Group E were given kolaviron orally at concentration of 200 mg/kg body weight once daily for 14 days after which clomiphene citrate were administered at concentration of 0.50 mg/kg body weight for 5 days; Group F were given kolaviron orally at concentration of 100 mg/kg body weight twice daily for 14 days after which clomiphene citrate were administered at concentration of 0.50 mg/kg body weight for 5 days. At the end of the experimental period, the animals were anaesthesized by chloroform inhalation. The ovary, fallopian tube and uterus were removed, weghed and fixed in 10% formol saline for Haematoxylin and Eosin staining. The results showed that Relative gonads weight (f=10.700, df=5, P<0.05) were significantly difference across the groups, The histoarchitechture of the ovary showed that kolaviron ingestion by the female wistar rats (groups B and C) resulted into drastical reduction if not total absence in the number of growing follicles, while uterus and fallopian tube revealed inflammed lumen, groups A and D were normal while groups E and F had evidence recovery from distortion. It was concluded that kolaviron have adverse effects on the ovary, fallopian tube and uterus in female wistar rats and this could add to its role as infertility agent while clomiphene citrate helped in their recovery.