High Profile Chlorpyrifos Degrading Pseudomonas putida MAS-1 from Indigenous Soil: Gas Chromatographic Analysis and Molecular Characterization

Abstract

Chlorpyrifos is an organophosphorous insecticide applied to soil to control pests in agricultural fields. It is well known important insecticide used for crop protection in Pakistan and its massively spread contamination is of logical concern. A number of water and land ecosystems may get polluted with organophosphorus compounds. These compounds have a significant level of toxicity for mammals including humans. It is thus essential to exclude these toxicants from the ecosystem as quickly as possible. The feasible approaches to achieve such goals include biodegradation and biomineralization (utilization). The study was designed to determine the potential of Ps. putida MAS-1 to degrade / utilize chlorpyrifos. Gas chromatography studies revealed that Ps. putida MAS-1 degraded chlorpyrifos rapidly. For the final identification of Ps. putida MAS-1 PCR amplification of V4 variable regions of 16S rDNA was done which showed band size of 876 bp. The research findings presented in this manuscript indicate that Pseudomonas putida MAS-1 can be exploited for the biodegradation of chlorpyrifos polluted environment.