Comparison of Different Parameters for the In Vitro Propagation of Various Cultivors of Olive (Olea Europaea L.)

Naveed Ahmad, Numan Khan, Fahad Ali, Khalil Ur Rahman, Sulaiman Bahadar, Muhammad Ismail Khan, Sami Ullah Jan, Raheem Bahadar et al

Abstract


Olive (Olea europaea L.) is most important fruit species and usually propagated by shoot and leaf cutting under the mist. By traditional methods the propagation of some fruit species is difficult to multiply and now make possible by micropropagation, regarding the olive micropropagation. In our study Uslu, Coratina, Nocellara, Pendolino, Leccino veriaty of Olive plants were taken sample were collected from its shoot. Then MS Media were used for inoculation along with growth regulators like BAP, NAA, GA3, coconut water. Then treat with Mancozeb + Metalaxyl and chlorox containing NaOCl to control the contamination. Then inoculate and the growth condition were apply. For fungal contamination, among all concentrations treatment with 7.5g/250mL Mancozeb for 1 hour prevented fungal contamination up to 90 %. For chlorox treatment, among all concentrations 20% chlorox treatment for 5 minutes was found to be the most effective to minimize browning and maximize sterilization. The growth regulator BAP, NAA and coconut water not show proliferation. And GA3 regenerate the plant growth. In light the plant produce more shoots than the dark one. Auxiliary buds have high regeneration capacity than the apical buds. Juivinail is batter then old explant in shoot sprouting.

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International Journal of Basic Medical Sciences and Pharmacy (IJBMSP): ISSN: 2049-4963